erich nigg (Addgene inc)
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erich nigg
Erich Nigg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erich+nigg/pcDNA3+Lats1+(Nigg+HS189)+(Plasmid+%2341156)/bio_rxiv__64898__2025__12__05__692666-276-33-35
Average 93 stars, based on 12 article reviews
Erich Nigg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erich+nigg/pcDNA3+Lats1+(Nigg+HS189)+(Plasmid+%2341156)/bio_rxiv__64898__2025__12__05__692666-276-33-35
Average 93 stars, based on 12 article reviews
erich nigg - by Bioz Stars,
2026-10
93/100 stars
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Imaging:Article Title: Nanotopography modulates cytoskeletal organization and dynamics during T cell activation Article Snippet: .. The supernatant was removed and the cells were resuspended in L-15 imaging medium. pEGFP Centrin2 (Nigg UK185) was a gift from Article Title: Nanotopography modulates cytoskeletal organization and dynamics during T cell activation Article Snippet: .. The supernatant was removed and the cells were resuspended in L-15 imaging medium. pEGFP Centrin2 (Nigg UK185) was a gift from Plasmid Preparation:Article Title: Nanotopography modulates cytoskeletal organization and dynamics during T cell activation Article Snippet: .. The supernatant was removed and the cells were resuspended in L-15 imaging medium. pEGFP Centrin2 (Nigg UK185) was a gift from Article Title: Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Article Snippet: .. CM1 domain from human CDK5RAP2 was amplified from pRcCMV-Cep215 (Nigg CW493) - a gift from Article Title: aPKC-ζ III promotes trophoblast fusion by altering Par-3 interactions with Hippo Signaling Kinase LATS1 Article Snippet: aPKC-ζ III-FLAG and aPKC-ζ III-EGFP plasmids were constructed and packaged by VectorBuilder (Table 4). .. Vector IDs can be used to retrieve detailed information about the vector on vectorbuilder.com. pEGFP-N1-Par3 plasmid was a gift from Dr. Masanori Nakayama. pcDNA3 Lats1 (Nigg HS189) (LATS1-Myc Tag) was a gift from Article Title: Nanotopography modulates cytoskeletal organization and dynamics during T cell activation Article Snippet: .. The supernatant was removed and the cells were resuspended in L-15 imaging medium. pEGFP Centrin2 (Nigg UK185) was a gift from Article Title: The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregation. Article Snippet: .. pcDNA-HA-MST2 was a gift from Kunliang Guan ( 28 ) (Addgene plasmid # 33098), pEGFP C3-Mst2 was a gift from Marius Sudol ( 29 ) (Addgene plasmid # 19056), pEGFP-C3-PLK4-3xFLAG was a gift from Michel Bornens ( 30 ) (Addgene plasmid # 69837), pEGFP Centrin2 ( 3 ) (Nigg UK185) was a gift from Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from Article Title: Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry. Article Snippet: HPV16 pL2-EGFP SSTP212AAAA, -S212A, -S213A, -T214A, -P215A, BPV1 pL2-EGFP TSTP239AAAA, -T241A, HPV18 pL2EGFP SSTP211AAAA, -T213A, and HPV5 pL2-EGFP T249A were generated from pL2-EGFP by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. .. For immunoprecipitations, HPV16 pL2-3xHA SSTP212AAAA, S212A, S213A, T214A, and P215A were generated from pL2-3xHA WT4 with mutagenic primers by using the QuikChange II XL Site-DirectedMutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. pcDNA3-Plk1(829) was a gift from Jonathon Pines (Addgene plasmid #39845). pcDNA3 PBD wt (Nigg AH12) was a gift from Amplification:Article Title: Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Article Snippet: .. CM1 domain from human CDK5RAP2 was amplified from pRcCMV-Cep215 (Nigg CW493) - a gift from Polymerase Chain Reaction:Article Title: Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain. Article Snippet: .. CM1 domain from human CDK5RAP2 was amplified from pRcCMV-Cep215 (Nigg CW493) - a gift from Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from Retroviral:Article Title: Polo-like kinase 1 (PLK1) is a novel CARD14-binding protein in keratinocytes. Article Snippet: .. For the retroviral transduction of HaCaT cells, the constructs pDG2i_hCARD14 E138A-FLAG puro (LMBP: 13397), pDG2i_hCARD14 E138A S473-475A-FLAG puro (LMBP: 13398) and pDG2i_hCARD14 S473475A-FLAG puro (LMBP: 13399) were generated via site-directed mutagenesis of the original construct pDG2i-hCARD14-FLAG-puro (LMBP: 9868). pRcCMV mycPlk1 wt (Nigg RG6) and pRcCMV myc-Plk1 K82R (Nigg LA184) were a gift from Transduction:Article Title: Polo-like kinase 1 (PLK1) is a novel CARD14-binding protein in keratinocytes. Article Snippet: .. For the retroviral transduction of HaCaT cells, the constructs pDG2i_hCARD14 E138A-FLAG puro (LMBP: 13397), pDG2i_hCARD14 E138A S473-475A-FLAG puro (LMBP: 13398) and pDG2i_hCARD14 S473475A-FLAG puro (LMBP: 13399) were generated via site-directed mutagenesis of the original construct pDG2i-hCARD14-FLAG-puro (LMBP: 9868). pRcCMV mycPlk1 wt (Nigg RG6) and pRcCMV myc-Plk1 K82R (Nigg LA184) were a gift from Construct:Article Title: Polo-like kinase 1 (PLK1) is a novel CARD14-binding protein in keratinocytes. Article Snippet: .. For the retroviral transduction of HaCaT cells, the constructs pDG2i_hCARD14 E138A-FLAG puro (LMBP: 13397), pDG2i_hCARD14 E138A S473-475A-FLAG puro (LMBP: 13398) and pDG2i_hCARD14 S473475A-FLAG puro (LMBP: 13399) were generated via site-directed mutagenesis of the original construct pDG2i-hCARD14-FLAG-puro (LMBP: 9868). pRcCMV mycPlk1 wt (Nigg RG6) and pRcCMV myc-Plk1 K82R (Nigg LA184) were a gift from Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from Generated:Article Title: Polo-like kinase 1 (PLK1) is a novel CARD14-binding protein in keratinocytes. Article Snippet: .. For the retroviral transduction of HaCaT cells, the constructs pDG2i_hCARD14 E138A-FLAG puro (LMBP: 13397), pDG2i_hCARD14 E138A S473-475A-FLAG puro (LMBP: 13398) and pDG2i_hCARD14 S473475A-FLAG puro (LMBP: 13399) were generated via site-directed mutagenesis of the original construct pDG2i-hCARD14-FLAG-puro (LMBP: 9868). pRcCMV mycPlk1 wt (Nigg RG6) and pRcCMV myc-Plk1 K82R (Nigg LA184) were a gift from Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from Article Title: Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry. Article Snippet: HPV16 pL2-EGFP SSTP212AAAA, -S212A, -S213A, -T214A, -P215A, BPV1 pL2-EGFP TSTP239AAAA, -T241A, HPV18 pL2EGFP SSTP211AAAA, -T213A, and HPV5 pL2-EGFP T249A were generated from pL2-EGFP by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. .. For immunoprecipitations, HPV16 pL2-3xHA SSTP212AAAA, S212A, S213A, T214A, and P215A were generated from pL2-3xHA WT4 with mutagenic primers by using the QuikChange II XL Site-DirectedMutagenesis Kit (Agilent Technologies) according to manufacturer’s instruction. pcDNA3-Plk1(829) was a gift from Jonathon Pines (Addgene plasmid #39845). pcDNA3 PBD wt (Nigg AH12) was a gift from Mutagenesis:Article Title: Polo-like kinase 1 (PLK1) is a novel CARD14-binding protein in keratinocytes. Article Snippet: .. For the retroviral transduction of HaCaT cells, the constructs pDG2i_hCARD14 E138A-FLAG puro (LMBP: 13397), pDG2i_hCARD14 E138A S473-475A-FLAG puro (LMBP: 13398) and pDG2i_hCARD14 S473475A-FLAG puro (LMBP: 13399) were generated via site-directed mutagenesis of the original construct pDG2i-hCARD14-FLAG-puro (LMBP: 9868). pRcCMV mycPlk1 wt (Nigg RG6) and pRcCMV myc-Plk1 K82R (Nigg LA184) were a gift from Purification:Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from Recombinant:Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from Expressing:Article Title: Partial closure of the γ-tubulin ring complex by CDK5RAP2 activates microtubule nucleation. Article Snippet: .. Purification of recombinant, untagged CDK5RAP2 fragments A bacterial expression construct for amino acids 44-93 of CDK5RAP2 lacking a GFP tag was generated by first PCR amplifying CDK5RAP244-93 from pRcCMV Cep215 (NCBI accession BAB13459.3), a gift from |